rabbit anti p creb s133  (Cell Signaling Technology Inc)

Journal: The Journal of Headache and Pain

Article Title: Dysregulated autophagy, neural signaling pathways and gut-brain axis in a novel vestibular migraine-like rat model: implications for pathogenesis

doi: 10.1186/s10194-025-02170-5

Figure Lengend Snippet: Activation of the NMDAR1-CaMKII-CREB pathway in different brain regions of VM-like rats. A Representative Western blot bands of NMDAR1-CaMKII-CREB pathway protein in the TCC, VN, thalamus and cerebellum. B - G Densitometric quantification of NMDAR1 ( A ), CAMKⅡ( B ), p- CAMKⅡ( C ), CREB ( D ), p-CREB ( E ), c-fos ( F ) in different brain regions of different groups: VM-like rats showed significant upregulation of NMDAR1, CaMKII, p-CaMKII, CREB, p-CREB, and c-Fos in the VN (vs. SHAM, p < 0.05). Similar elevations (except for p-CREB) were observed in the TCC and thalamus (vs. SHAM, p < 0.05), no obvious activation of the pathway was detected in the cerebellum of VM-like rats. ( n = 6/group, Results are mean ± SD, One-way ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: p-CREB-s133 , Abclonal, China , AP0019 , Rabbit , 1:2000.

Techniques: Activation Assay, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: The transcription factor CREB regulates epithelial-mesenchymal transition of lens epithelial cells by phosphorylation-dependent and phosphorylation-independent mechanisms

doi: 10.1016/j.jbc.2024.108064

Figure Lengend Snippet: CREB S133 phosphorylation and CBP interaction mediate mesenchymal genes expression in lens epithelial cells. A , αTN4-1 cells were treated with 20 ng/ml TGFβ for 24 h, and Western blot analysis was used to examine the protein expression levels of p-CREB S133, Fibronectin, and SNAI2 in mock or TGFβ-treated αTN4-1 cells. B , quantification results of Fibronectin, SNAI2, and p-CREB S133 protein levels in A . C , the anterior capsules of one lens of the mouse was punctured with a needle to induce ASC, and the other lens remains as untreated normal. After 3 days of healing, the protein expression levels of lens capsular epithelium from normal and injured-induced ASC mice were examined. Western blot analysis was used to examine the protein expression levels of p-CREB S133 and Fibronectin in the lens epithelium of the normal and injured-induced ASC mice. D , quantification results of Fibronectin and p-CREB S133 protein levels in ( C ). E , immunofluorescence were used to analyze p-CREB S133 ( green ), α-SMA ( red ), and lens epithelial cell nuclei in the lens capsule of the normal and injured-induced ASC mice ( blue ) (n = 3 lenses per group). two-tailed Student’s t test was used in ( B ) and ( D ), The data shown are the averages of three independent biological experiments, and error bars represent the SD of mean. ∗∗ p < 0.01, ∗ p < 0.05, n.s, statistically not significant.

Article Snippet: The sources and dilutions of antibodies were as follows: rabbit anti-Fibronectin (1:1000, Abcam #23750), rabbit anti-SNAI2 (1:1000, Cell Signaling Technology #9585), rabbit anti-CREB (1:1000, Cell Signaling Technology #9197), rabbit anti-p-CREB S133 (1:1000, Cell Signaling Technology #9198), rabbit anti-CBP (1:1000, Cell Signaling Technology #7389), rabbit anti-p300 (1:1000, Cell Signaling Technology #70088), mouse anti-β-Tubulin (1:5000, proteintech, 66240-1-Ig), rabbit anti-α-Actinin(1:5000, proteintech, 11313-2-AP), horseradish peroxidase–conjugated horse anti-rabbit/mouse IgG (1:5000, Cell Signaling Technology).

Techniques: Phospho-proteomics, Expressing, Western Blot, Capsules, Immunofluorescence, Two Tailed Test